Bst DNA Polymerase

Heat-resistant strand displacement DNA polymerase
Top> Reagents for research> Bst DNA Polymerase
Product Name Code No. Size Price Note
Bst DNA Polymerase
311-07481
1,600 units
   

Product Description

This product is an enzyme having heat-resistant property and a strand-displacement type DNA polymerase activity, which synthesizes a new DNA strand while dissociating the hydrogen bond of the double stranded template DNA by itself. Since the strand-displacement DNA polymerase does not require dissociation of double-stranded DNA by its characteristics, DNA can be synthesized at a constant temperature, and the synthesis is not inhibited by the secondary structure of DNA.

Characteristics

  1. 5'→3' DNA polymerase activity and strand-displacement activity.
  2. DNA synthesis can be performed at a constant temperature.
  3. Suitable for synthesis of DNA strands having high GC content
  4. Not affected by inhibition due to the secondary structure of DNA
  5. High purity/high quality control

Applications

Bst DNA Polymerase
Optimum reaction temperature
60°C - 65°C
Deactivation temperature*1
80°C, for 5 min.

*1 Deactivation temperature when the stock enzyme solution is directly heat-denatured.

Source Geobacillus stearothermophilus
Concentration 8 units/µl
Unit Definition One unit is defined as the enzyme activity incorporating 10 nmoles of deoxynucleotide into acid-insoluble precipitates at 65°C in 30 min using activated calf thymus DNA as a primer/template.
Storage Conditions 10 mmol/l Tris-HCl (pH 7.5), 1 mmol/l DTT, 0.1% Tween 20, 0.1 mmol/l EDTA, 50% Glycerol
Reaction Conditions 1 x Bst Reaction Buffer

▲ Page Top

Product Components

Bst DNA Polymerase (1,600 units)
Component Quantity Storage Description
Bst DNA Polymerase (8 units/µl)
200 µl x 1 -20°C -
10 x Bst Reaction Buffer (80 mmol/l Mg2+) 500 µl x 1 -20°C -

▲ Page Top

Usage

Usage example: Application for LAMP method


3.0% Agarose 21 / TAE gel electrophoresis,
EtBr staining

M: Gene Ladder Wide 1 (Code No. 313-06961)
1: Bst DNA Polymerase of A company
2: Bst DNA Polymerase of Nippon Gene

LAMP method, Composition of the reaction liquid
Candidatus Liberibacter asiaticus DNA
1 x 106 copies
FIP*
40 pmol
BIP*
40 pmol
F3 Primer*
5 pmol
B3 Primer*
5 pmol
Loop Primer F*
20 pmol
Loop Primer B*
20 pmol
dNTPs Mixture
1.4 mM each
10 x Bst Reaction Buffer
2.5 µl
Bst DNA Polymerase
8 units
Total
25 µl

Reaction
65°C 60 min.

* Primer Sequence
  FIP: 5'-GCATGCCGAGGATCAATGCCTTGCTTAAAGAGCGTGCTACG-3'
BIP: 5'-TATGCCTAATGGCACGGGGGTAAGCTTCATCCGCCTTCGA-3'
F3 Primer: 5'-TGGGTTAAGTGATGCTGTGG-3'
B3 Primer: 5'-CAACAATATCAGCCCCTGCT-3'
Loop Primer F: 5'-TCTCAACTGTTTCATCAAACCTAGC-3'
Loop Primer B: 5'- CGTGGCGGTTTTTGCTACA-3'

▲ Page Top

Data Sheets

SDS(Safety Data Sheet)

▲ Page Top

Related Information

Note

License

Related Products

Contact us

Distributor
FUJIFILM Wako Pure Chemical Corporation
Produced by
NIPPON GENE CO., LTD.

ページトップ