ISOGEN and ISOGEN-LS

Isolation of total RNA
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Product Name Code No. Size Price Note
ISOGEN
315-02504
10 ml
  (phenol formulations)
ISOGEN
317-02503
50 ml
  (phenol formulations)
ISOGEN
311-02501
100 ml
  (phenol formulations)
ISOGEN-LS
317-02623
10 ml
  (phenol formulations)
ISOGEN-LS
311-02621
100 ml
  (phenol formulations)

Product Description

ISOGEN and ISOGEN-LS are reagents for RNA extraction from humans, animals, plants and bacteria. The liquid phase separation method is used, and DNA and protein can be isolated from a single sample. Since RNA, DNA and protein can be isolated through a series of manipulations, it is very effective for analyzing precious samples, and the simplicity of the manipulations allows a large number of samples to be handled.

ISOGEN and ISOGEN-LS are homogenous liquids containing phenol and guanidine thiocyanate, with ISOGEN-LS containing more phenol than ISOGEN. ISOGEN is used for the samples of tissues and cultured cells, and ISOGEN-LS is effectively used for samples of large volume such as blood (> 100 µl when processed in a 1.5 ml plastic tube) and liquid samples that are difficult to concentrate.

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Product Components

ISOGEN (100 ml)
Component Quantity Storage Description
ISOGEN 100 ml x 1 +4°C blue solution (phenol formulations)
ISOGEN-LS (100 ml)
Component Quantity Storage Description
ISOGEN-LS 100 ml x 1 +4°C red solution (phenol formulations)

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Usage

Precautions

Plotocol

ISOGEN and ISOGEN-LS are homogenous liquids containing phenol and guanidine thiocyanate, with ISOGEN-LS containing more phenol than ISOGEN. ISOGEN is used for the samples of tissues and cultured cells, and ISOGEN-LS is effectively used for samples of large volume such as blood (> 100 µl when processed in a 1.5 ml plastic tube) and liquid samples that are difficult to concentrate.

ISOGEN or ISOGEN-LS is added to a sample, and after dissolving or homogenizing, chloroform is added to the mixture and centrifuged. The homogenate is separated into 3 phases: an aqueous phase, an organic phase and an intermediate phase. In the aqueous phase, only RNA is present, and DNA and protein are present in the intermediate phase and the lower phase. The aqueous phase is collected, and RNA is precipitated by adding isopropanol. DNA is precipitated by adding ethanol to the residual intermediate phase and organic phase, and protein is then precipitated by adding isopropanol. In this way, RNA, DNA and protein can be sequentially isolated.

protocol

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Data Sheets

Technical Data Sheet

SDS(Safety Data Sheet)

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Related Information

Note

Reference

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FUJIFILM Wako Pure Chemical Corporation
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NIPPON GENE CO., LTD.

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