Gene Ladder

pre-mixed, ready-to-load DNA markers
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Product Name Code No. Size Price Note
Gene Ladder 100 (0.1-2 kbp)
 316-06951
500 µl (100 lanes)
    
Gene Ladder Wide 1 (0.1-20 kbp) 313-06961
500 µl x 2 (200 lanes)
    
Gene Ladder Wide 2 (0.1-20 kbp) 310-06971
500 µl x 2 (200 lanes)
    
Gene Ladder Fast 1 (0.1-2 kbp) 317-06981
500 µl x 2 (200 lanes)
    
Gene Ladder Fast 2 (0.1-10 kbp) 314-06991
500 µl x 2 (200 lanes)
    

Product Description

Gene Ladder is a ladder marker to which dyes (Bromophenol blue, Xylene Cyanol FF) and Glycerol (for increasing density) are added beforehand, and can be applied to agarose gel as-is.
Gene Ladder is used at 5 µl per lane and can be used as a mass marker by staining with ethidium bromide or the like after electrophoresis.

Storage Conditions 10 mmol/l Tris-HCl(pH 8.0), 10 mmol/l EDTA, 20 mmol/l NaCl, 0.004%(w/v) Bromophenol blue, 0.004%(w/v) Xylene Cyanol FF, 10%(v/v) Glycerol
Marker Concentration Gene Ladder 100 (0.1-2 kbp): 0.64 µg/5 µl
Gene Ladder Wide 1 (0.1-20 kbp): 0.886 µg/5 µl
Gene Ladder Wide 2 (0.1-20 kbp): 0.925 µg/5 µl
Gene Ladder Fast 1 (0.1-2 kbp): 0.1 µg/5 µl
Gene Ladder Fast 2 (0.1-10 kbp): 0.15 µg/5 µl
Usage Apply 5 µl of Gene Ladder per agarose-gel lane.
When used as a mass marker, stain the gel after electrophoresis.
Storage Room temperature (for long-term storage, store at 4°C)

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Usage

Gene Ladder 100 (0.1-2 kbp)

This product is a DNA size and mass marker covering 100 bp-2,000 bp. A ladder is formed from 100 bp to 1,000 bp for each 100 bp, and since the DNA quantity of the short chain band is increased for better visibility, this product is best suited for the analysis of low molecular weightregion.
This product contains dyes and glycerol (for increasing density) and can be applied directly from the tube to an agarose gel as-is. Since the band pattern is simple and the 100 bp band is easy to recognize, this product is strongly recommend to those who are working in the low molecular weight region.

Fragment Base Pairs (bp) Mass
(ng/5 µl)

onestep-marker1
1 2,000 100
2 1,500 75
3 1,000 50
4 900 45
5 800 40
6 700 35
7 600 30
8 500 75
9 400 40
10 300 30
11 200 20
12 100 100

 

Gene Ladder Wide (0.1-20 kbp)

This product is a DNA size and mass marker suitable for eparating in the range of 100 bp-20 kbp.
This product is recommended to those who need a ladder marker covering a wide range.

gene-ladder-wide
Gene Ladder Wide 1
(0.1-20 kbp)		   Gene Ladder Wide 2
(0.1-20 kbp)
Base pairs
(bp)		 Mass
(ng/5 µl)		 Fragment Base pairs
(bp)		 Mass
(ng/5 µl)
20,000 100 1 20,000 100
10,000 50 2 10,000 50
7,000 35 3 7,000 35
5,000 75 4 5,000 75
4,000 40 5 4,000 40
3,000 30 6 3,000 30
2,000 100 7 2,000 100
1,500 30 8 1,500 30
1,300 26 9 1,000 50
1,000 100 10 900 45
700 35 11 800 40
500 75 12 700 35
400 40 13 600 30
300 30 14 500 75
200 20 15 400 40
100 100 16 300 30
    17 200 20
    18 100 100

 

Gene Ladder Fast

Each of these products contains small number of bands (4 or 6), and the bands are separated in a short electrophoresis run (10 min). Thus these DNA ladder markers are most suited for a simplified check. This product is recommended to those who want to know the electrophoresis results rapidly, e.g., for colony PCR.

gene-ladder-wide
Gene Ladder Fast 1
(0.1-2 kbp)		   Gene Ladder Fast 2
(0.1-10 kbp)
Base pairs
(bp)		 Mass
(ng/5 µl)		 Fragment Base pairs
(bp)		 Mass
(ng/5 µl)
2,000 25 1 10,000 25
1,000 25 2 4,000 25
500 25 3 2,000 25
100 25 4 1,000 25
    5 500 25
    6 100 25

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Data Sheets

SDS(Safety Data Sheet)

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Related Information

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Distributor
FUJIFILM Wako Pure Chemical Corporation
Produced by
NIPPON GENE CO., LTD.
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