Agarose S
Agarose for Gel Electrophoresis| Product Name | Code No. | Size | Price | Note |
|---|---|---|---|---|
Agarose S |
316-01191 |
5 g |
||
Agarose S |
312-01193 |
100 g |
||
Agarose S |
318-01195 |
500 g |
||
Agarose S |
313-90231 |
1 kg |
Product Description
Agarose S is specially purified for separating and recovering DNA fragments.
Features
・low EEO・DNase free, RNase free・Recommended Gel Concentration: 0.8 - 2 %・Nucleic Acid Fragment Separation Range: 0.5 - 30 kbp
Applications
- Gel Electrophoresis for separation of DNA fragments
- Pulsed-Field Electrophoresis (1 - 2,000 kbp)
- Recovered DNA*1
- Blotting
*1 Recovered DNA from preparative Agarose gels is checked that it can be used for downstream applications (cleavage by restriction endonucleases, ligation by T4 DNA ligase, E. coli transformation by ligated DNA.)
| Gel strength | ≥ 1,200 g/cm2 (1.5%) |
|---|---|
| Melting point | ≤ 90°C (1.5%) |
| Gelling temperature | 37 - 39°C (1.5%) |
| Sulfate content (SO4) | ≤ 0.1% |
| Water content | ≤ 10% |
| Electroendosmosis (-Mr) | ≤ 0.1 (Low EEO) |
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Usage
Separation of DNA markers in Agarose S
Agarose S 1% Gel
| Lane | 1 |
2 |
3 |
4 |
| λ Marker | Marker 1 | Marker 2 | Marker 3 | Marker 6 |
| DNA fragments (kbp) |
23.13 9.42 6.56 4.36 2.32 2.02 0.56 0.13 |
21.23 5.15 4.98 4.27 3.52 2.02 1.91 1.58 1.38 0.95 0.83 0.56 0.13 |
23.13 21.23 9.42 7.42 6.56 5.81 5.65 4.88 4.36 3.52 2.32 2.02 0.56 0.13 |
19.33 7.74 6.22 4.26 3.47 2.69 1.88 1.49 0.93 0.42 0.07 |
Effect of Agarose concentration on the migration distance of bands
Molecular weight markers were electrophoresed using Agarose S 0.5% gel, 1% gel and 2% gel, and the effect of the Agarose gel concentration on the migration distance of the band was investigated. (Use as a reference for determining the concentration when a gel is prepared.)
Ladder Markers
Lane 1：OneSTEP Ladder 1 kb
Lane 2：OneSTEP Ladder 500
Lane 3：OneSTEP Ladder 100
Lane 4：Smart Ladder
Lane 5：OneSTEP Marker 1 (λ/Hind III digest)
Lane 6：OneSTEP Marker 6 (λ/Sty I digest)
Lane 7：OneSTEP Marker 4 (φX174/Hae III digest)
Lane 8：OneSTEP Marker 5 (φX174/Hinc II digest )
0.5% Agarose S |
1% Agarose S |
2% Agarose S |
 |
 |
 |
| Lane | 1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
| DNA fragments (kbp) |
10.0 9.0 8.0 7.0 6.0 5.0 4.0 3.0 2.0 1.0 |
5.0 4.5 4.0 3.5 3.0 2.5 2.0 1.5 1.0 0.5 |
2.0 1.8 1.6 1.4 1.2 1.0 0.9 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 |
10.0 8.0 6.0 5.0 4.0 3.0 2.5 2.0 1.5 1.0 0.8 0.6 0.4 0.2 |
23.1 9.4 6.6 4.4 2.3 2.0 0.6 0.1 |
19.3 7.7 6.2 4.3 3.5 2.7 1.9 1.5 0.9 0.4 0.07 |
1.35 1.08 0.87 0.60 0.31 0.28 0.27 0.23 0.19 0.12 0.07 |
1.057 0.770 0.612 0.495 0.392 0.345 0.341 0.335 0.297 0.291 0.210 0.162 0.079 |
Recommended Gel Concentration
- 1 % Agarose S gel (greater than or equal to 1 kb DNA)
- 2 % Agarose S gel (less than 1 kb DNA)
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Data Sheets
SDS(Safety Data Sheet)
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Related Information
Note
- This product is for research use only.
Contact us
- Distributor
- FUJIFILM Wako Pure Chemical Corporation
- Produced by
- NIPPON GENE CO., LTD.
- E-mail：Web Contact Form
