Gene Taq NT

Taq DNA polymerase
Top> Reagents for research> Gene Taq NT
Product Name Code No. Size Price Note
Gene Taq NT
312-03234
50 units
   
Gene Taq NT
318-03231
250 units
   
Gene Taq NT
314-03233
250 units x 4
   

Product Description

Gene Taq NT is a heat-resistant DNA polymerase which is obtained by cloning the DNA polymerase gene of Thermus aquaticus YT1, expressing in Escherichia coli, and separating and purifying. This has the same function as the natural Taq DNA polymerase and can be used for normal PCR.
Also, having a terminal transferase activity, the PCR product obtained can be used for TA cloning.

Applications

Molecular Weight 94 kDa
Concentration 5 units/μl
Storage Conditions 20 mmol/l Tris-HCl (pH 8.0), 100 mmol/l KCl, 0.1 mmol/l EDTA, 0.5% Tween 20, 1 mmol/l DTT, 50% Glycerol
Unit Definition One unit is defined as the enzyme activity incorporating 10 nmoles of deoxynucleotide into acid-insoluble precipitates at 74°C for 30 min using activated calf thymus DNA as a primer/template.
Purity No change was observed in the agarose gel electrophoresis pattern of DNA after reacting 10 units of this enzyme and 1 µg of λ/Hind III digest at 74°C for 1 hr.

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Product Components

Gene Taq NT (50 units)
Component Quantity Storage Description
Gene Taq NT 50 units x 1 -20°C  
dNTP Mixture (2.5 mmol/l each) 160 μl x 1 -20°C  
10 x Gene Taq Universal Buffer (15 mmol/l Mg2+) 1 ml x 1 -20°C  
Gene Taq NT (250 units)
Component Quantity Storage Description
Gene Taq NT 250 units x 1 -20°C  
dNTP Mixture (2.5 mmol/l each) 800 μl x 1 -20°C  
10 x Gene Taq Universal Buffer (15 mmol/l Mg2+) 1 ml x 1 -20°C  

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Data Sheets

SDS(Safety Data Sheet)

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Related Information

Note

Related Products

Comparison of Gene Taq series
Product Characteristics 5' → 3' exonuclease activity terminal transferase activity
Gene Taq The amplification efficiency of DNA fragments not more than 1 kbp is especially high.
Gene Taq NT Gene Taq NT has the same functions as natural Taq DNA polymerase.
Gene Taq FP Gene Taq FP obtained by further purifying Gene Taq to a higher degree of purity and by controlling the contamination by the host-derived DNA as much as possible. It is suitable for RAPD PCR which is affected by contamination by the genomic DNA.

Contact us

Distributor
FUJIFILM Wako Pure Chemical Corporation
Produced by
NIPPON GENE CO., LTD.

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