Agarose S

Agarose for Gel Electrophoresis
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Product Name Code No. Size Price Note
Agarose S
316-01191
5 g
   
Agarose S
312-01193
100 g
   
Agarose S
318-01195
500 g
   
Agarose S
313-90231
1 kg
   

Product Description

Agarose S is specially purified for separating and recovering DNA fragments.

Features

・low EEO・DNase free, RNase free・Recommended Gel Concentration: 0.8 - 2 %・Nucleic Acid Fragment Separation Range: 0.5 - 30 kbp

Applications

*1 Recovered DNA from preparative Agarose gels is checked that it can be used for downstream applications (cleavage by restriction endonucleases, ligation by T4 DNA ligase, E. coli transformation by ligated DNA.)

Specification
Gel strength
≥ 1,200 g/cm2 (1.5%)
Melting point
≤ 90°C (1.5%)
Gelling temperature
37 - 39°C (1.5%)
Sulfate content (SO4)
≤ 0.1%
Water content
≤ 10%
Electroendosmosis (-Mr)
≤ 0.1 (Low EEO)

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Usage

Separation of DNA markers in Agarose S


Agarose S 1% Gel

 
Lane
1
2
3
4
λ Marker Marker 1 Marker 2 Marker 3 Marker 6
DNA fragments
(kbp)

23.13

9.42

6.56

4.36

2.32

2.02

0.56

0.13

21.23

5.15

4.98

4.27

3.52

2.02

1.91

1.58

1.38

0.95

0.83

0.56

0.13

23.13

21.23

9.42

7.42

6.56

5.81

5.65

4.88

4.36

3.52

2.32

2.02

0.56

0.13

19.33

7.74

6.22

4.26

3.47

2.69

1.88

1.49

0.93

0.42

0.07


Effect of Agarose concentration on the migration distance of bands

Molecular weight markers were electrophoresed using Agarose S 0.5% gel, 1% gel and 2% gel, and the effect of the Agarose gel concentration on the migration distance of the band was investigated. (Use as a reference for determining the concentration when a gel is prepared.)

Ladder Markers

Lane 1:OneSTEP Ladder 1 kb
Lane 2:OneSTEP Ladder 500
Lane 3:OneSTEP Ladder 100
Lane 4:Smart Ladder
Lane 5:OneSTEP Marker 1 (λ/Hind III digest)
Lane 6:OneSTEP Marker 6 (λ/Sty I digest)
Lane 7:OneSTEP Marker 4 (φX174/Hae III digest)
Lane 8:OneSTEP Marker 5 (φX174/Hinc II digest )

0.5% Agarose S
1% Agarose S
2% Agarose S
agarose-s_1% agarose-s_2%
Lane
1
2
3
4
5
6
7
8
DNA fragments
(kbp)
10.0
9.0
8.0
7.0
6.0
5.0
4.0
3.0
2.0
1.0
5.0
4.5
4.0
3.5
3.0
2.5
2.0
1.5
1.0
0.5
2.0
1.8
1.6
1.4
1.2
1.0
0.9
0.8
0.7
0.6
0.5
0.4
0.3
0.2
0.1
10.0
8.0
6.0
5.0
4.0
3.0
2.5
2.0
1.5
1.0
0.8
0.6
0.4
0.2
23.1
9.4
6.6
4.4
2.3
2.0
0.6
0.1
19.3
7.7
6.2
4.3
3.5
2.7
1.9
1.5
0.9
0.4
0.07
1.35
1.08
0.87
0.60
0.31
0.28
0.27
0.23
0.19
0.12
0.07
1.057
0.770
0.612
0.495
0.392
0.345
0.341
0.335
0.297
0.291
0.210
0.162
0.079

Recommended Gel Concentration

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Data Sheets

SDS(Safety Data Sheet)

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Related Information

Note

Related Products

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Distributor
FUJIFILM Wako Pure Chemical Corporation
Produced by
NIPPON GENE CO., LTD.

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