Gene Taq

Taq DNA polymerase
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Product Name Code No. Size Price Note
Gene Taq
312-02874
50 units
   
Gene Taq
318-02871
250 units
   
Gene Taq
314-02873
250 units x 4
   

Product Description

Gene Taq is a heat-resistant DNA polymerase which is obtained by cloning the DNA polymerase gene of Thermus aquaticus YT1, modifying the gene, expressing it in Escherichia coli, and separating and purifying. Gene Taq is suitable for PCR. Also, having a terminal transferase activity, the PCR product obtained can be used for TA cloning. Since the N-terminal of Taq DNA polymerase is partially deleted, the 5'-3' exonuclease activity is lost.
Also, the amplification efficiency for DNA fragments of not more than 1 kbp is especially high.

Applications

Molecular Weight 68 kDa
Concentration 5 units/μl
Storage Conditions 20 mmol/l Tris-HCl (pH 8.0), 100 mmol/l KCl, 0.1 mmol/l EDTA, 0.5% Tween 20, 1 mmol/l DTT, 50% Glycerol
Unit Definition One unit is defined as the enzyme activity incorporating 10 nmoles of deoxynucleotide into acid-insoluble precipitates at 74°C for 30 min using activated calf thymus DNA as a primer/template.
Purity No change was observed in the agarose gel electrophoresis pattern of DNA after reacting 10 units of this enzyme and 1 µg of λ/Hind III digest at 74°C for 1 hr.

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Product Components

Gene Taq (50 units)
Component Quantity Storage Description
Gene Taq 50 units x 1 -20°C  
dNTP Mixture (2.5 mmol/l each) 160 μl x 1 -20°C  
10 x Gene Taq Universal Buffer (15 mmol/l Mg2+) 1 ml x 1 -20°C  
Gene Taq (250 units)
Component Quantity Storage Description
Gene Taq 250 units x 1 -20°C  
dNTP Mixture (2.5 mmol/l each) 800 μl x 1 -20°C  
10 x Gene Taq Universal Buffer (15 mmol/l Mg2+) 1 ml x 1 -20°C  

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Data Sheets

SDS(Safety Data Sheet)

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Related Information

Note

Related Products

Comparison of Gene Taq series
Product Characteristics 5' → 3' exonuclease activity terminal transferase activity
Gene Taq The amplification efficiency of DNA fragments not more than 1 kbp is especially high.
Gene Taq NT Gene Taq NT has the same functions as natural Taq DNA polymerase.
Gene Taq FP Gene Taq FP obtained by further purifying Gene Taq to a higher degree of purity and by controlling the contamination by the host-derived DNA as much as possible. It is suitable for RAPD PCR which is affected by contamination by the genomic DNA.

Contact us

Distributor
FUJIFILM Wako Pure Chemical Corporation
Produced by
NIPPON GENE CO., LTD.

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