T4 gene 32 protein

a nucleic acid binding protein

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Product Name	Code No.	Size	Price	Note
T4 gene 32 protein	318-03253	20 µg
T4 gene 32 protein	312-03251	100 µg

Product Description

T4 gene 32 protein is a nucleic acid binding protein that is essential for bacteriophage T4 DNA and has the function of destabilizing the helical structure. It is used for promoting DNA synthesis by DNA polymerase, site-specific mutagenesis, eliminating extra bands during sequencing^(Ref.5, marking single stranded DNA for electron microscope and the like. Its activity in reducing the effect of inhibitory substances in PCR is also noted.

Source	Escherichia coli BB infected with T4（33^- 55^- 61^-）phage
Concentration	3 - 8 mg/ml
Storage Conditions	20 mmol/l Tris-HCI(pH 8.1), 100 mmol/l NaCl, 1 mmol/l EDTA, 1 mmol/l DTT, 50%(v/v) Glycerol
Purity	No change was observed in the electrophoresis pattern of λDNA after reacting 50 µg of this enzyme and 1 µg of λDNA at 37°C for 1 hr.

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Product Components

T4 gene 32 protein (20 µg)
Component	Quantity	Storage	Description
T4 gene 32 protein	20 µg	-20°C

T4 gene 32 protein (100 µg)
Component	Quantity	Storage	Description
T4 gene 32 protein	100 µg	-20°C

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Usage

Reduction of PCR inhibition by hemin using T4 gene 32 protein

PCRs were performed in reaction solutions containing 0.1 ng template DNA (pBR322) and hemin at a series of concentrations in the presence or absence of T4 gene 32 protein.

Composition of the reaction liquid
template DNA (pBR322)	0.1 ng
hemin	final concentration 10 - 0.001 µg/µl
10 x Gene Taq Universal Buffer	5 µl
dNTP Mixture (2.5mmol/l each)	4 µl
primer-forward (20 pmol/µl)	1 µl
primer-reverse (20 pmol/µl)	1 µl
Gene Taq (5 units/µl)	0.5 µl
T4 gene 32 protein	0 or 2 µg (final concentration 40 ng/µl)
ddH2O	up to 50 µl

PCR
94°C	30 sec.	25 cycles
55°C	30 sec.
72°C	1 min.

Electrophoresis (10 µl)

2% Agarose S 100V, 30 min., EtBr Staining M：Marker 5 (φX174/Hinc ll)

Results

In the presence of hemin at a concentration of 1 µg/µl, PCR is inhibited, but when 40 ng/µl of T4 gene 32 protein (gp32) is added, the inhibition by 1 µg/µl of hemin is reduced.

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Data Sheets

SDS(Safety Data Sheet)

Please go to the page in Japanese.

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Related Information

Note

This product is for research use only.

References

Kreader, C. A. : Appl. Environ. Microbiol., 62 (3), 1102 (1996)
Bittner, M., Burke, R. L. and Alberts, B. M. : J. Biol. Chem., 254 (19), 9565 (1979)
Schwarz, K., Hansen-Hagge, T. and Bartram, C. : Nucleic Acids Res., 18 (4), 1079 (1990)
Panaccio, M. and Lew, A. : Nucleic Acids Res., 19 (5), 1151 (1991)
Kaspar, P., Zadrazil, S.and Fabry, M. : Nucleic Acids Res., 17 (9), 3616 (1989)
Huang, H. and Keohavong, P. : DNA AND CELL BIOLOGY, 15 (7), 589 (1996)

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