Charomid DNA

cosmid vector

Product Name	Code No.	Size
Charomid 9-20	311-01381	10 µg
Charomid 9-28	318-01391	10 µg
Charomid 9-36	311-01401	10 µg
Charomid 9-42	318-01411	10 µg

Product Description

Charomid vectors are useful for the construction of gene and genomic libraries.

Since Charomid vectors can be packaged in vitro, the cloning efficiency is comparable to that of lambda vectors and is high.
Charomid vectors have the 9 cloning sites. (Aat II, EcoR I, Sac I, Kpn I, Sma I, BamH I, Xba I, Apa I, Hind III)
After isolation of a clone, the vector size can be reduced to 2.9kb by deletion of the spacer sequences.
Restriction maps of the insert can be determined by a simple and rapid procedure.
For Charomid vectors are of 28kb and 36kb, there is no need to prepare vector arms, as is necessary for some lambda vectors, and partial digestion should not increase the number of clones without inserts.
Restriction patterns of recombinant Charomid vectors are very simple.

Source	Charomid is isolated from E. coli DH1.
Storage Conditions	10 mmol/l Tris-HCl(pH 8.0), 1 mmol/l EDTA
Concentration	0.2 - 0.8 µg/µl
Purity	A260/A280≒1.8 (This value is slightly different for each lot.)
Notes	Imperial Cancer Research Fund have sole ownership of Charomid vectors.

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Charomid DNA (10 µg)
Component	Quantity	Storage	Description
Charomid DNA	10 µg	-20°C	-

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Map
Amp^r; ampicillin tolerance gene, cos; cos region, ori; replication origin

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