ISOPLANT使用例 (放線菌、カビからのDNA抽出)


1. 抽出DNAの収量と純度

yeild (µg/g) A260/280
Mycobacterium phlei
10.8
1.79
Nocardia asteroides
21.2
1.92
Streptomyces albus
3.3
2.22
Streptomyces sp. 2-1
0.80
2.00
Streptomyces sp. MY-31
8.00
2.09
Neurospora sitophila
34.8
1.73
Rhizopus nigricans
23.0
2.04
Penicillium chrysogenum Q176
32.8
1.80
Aspergillus awamori
36.1
1.62
Aspergillus japonicus
20.5
1.90
Aspergillus niger
36.0
1.73
Aspergillus oryzae
6.8
2.04

example1photo lane 1. Mycobacterium phlei
lane 2. Nocardia asteroides
lane 3. Streptomyces albus
lane 4. Streptomyces sp. 2-1
lane 5. Streptomyces sp. MY-31
lane 6. Neurospora sitophila
lane 7. Rhizopus nigricans
lane 8. Penicillium chrysogenum Q176
lane 9. Aspergillus awamori
lane 10. Aspergillus japonicus
lane 11. Aspergillus niger
lane 12. Aspergillus oryzae
M : Marker 6 (λ/Sty I digest)

植物からのDNAの抽出
細菌からのDNAの抽出
酵母からのDNAの抽出


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